Some Early Experiments that Suggested the Feasibility of Amphibian Cloning
Blastula Nuclei Transplanted
The Transplantation of Nuclei Obtained from Embryos beyond that Blastula Stage, Rana pipens
Transplantation of Nuclei Obtained from Tumors of Rana pipens
The Transplantation of Nuclei Obtained from Embryos beyond the Blastula Stage: Xenopu laevis, Urodeles, and Some Studies from Russia and China
The Cloning of Adults and Other Transfers of Mature Nuclei
An Omnibus Chapter: A Discussion of Nuclear Transfer Studies Not Relating Directly to Nuclear Differentiation
Appendixes
In this, the first published monograph devoted exclusively to the cloning procedure, Professor McKinnel reviews the results obtained in nuclear transplantation experiments with amphibia and provides an extensive discussion of the methodology used. He explains that while biologists generally use the word “cloning” to refer to the production of multiple genetically identical individuals, he uses it in a more general sense to refer to one or more individuals produced by nuclear transplantation. He points out that results obtained from the cloning technique are often oversimplified and are sometimes misleading, and he discusses conditions which may lead to success or failure in achieving cloning. The extensive section on methodology describes, sufficiently to instruct new comers to the technique, the preparation of microscopic tools, micromanipulation procedure, the husbandry and reproductive biology of amphibians, dissociation of donor cells, and the activation and enucleation of mature ova. The work is generously illustrated with halftones and line drawings.